Quantitative transcriptional analysis of the genes encoding glycoside hydrolase family 7 cellulase isozymes in the basidiomycete Phanerochaete chrysosporium.
Identifieur interne : 000607 ( Main/Exploration ); précédent : 000606; suivant : 000608Quantitative transcriptional analysis of the genes encoding glycoside hydrolase family 7 cellulase isozymes in the basidiomycete Phanerochaete chrysosporium.
Auteurs : Hitoshi Suzuki [Japon] ; Kiyohiko Igarashi ; Masahiro SamejimaSource :
- FEMS microbiology letters [ 1574-6968 ] ; 2009.
Descripteurs français
- KwdFr :
- Amorces ADN (génétique), Analyse de profil d'expression de gènes (MeSH), Cellulase (biosynthèse), Cellulase (génétique), Isoenzymes (biosynthèse), Isoenzymes (génétique), Phanerochaete (enzymologie), Phanerochaete (génétique), RT-PCR (méthodes), Régulation de l'expression des gènes fongiques (MeSH).
- MESH :
- biosynthèse : Cellulase, Isoenzymes.
- enzymologie : Phanerochaete.
- génétique : Amorces ADN, Cellulase, Isoenzymes, Phanerochaete.
- méthodes : RT-PCR.
- Analyse de profil d'expression de gènes, Régulation de l'expression des gènes fongiques.
English descriptors
- KwdEn :
- Cellulase (biosynthesis), Cellulase (genetics), DNA Primers (genetics), Gene Expression Profiling (MeSH), Gene Expression Regulation, Fungal (MeSH), Isoenzymes (biosynthesis), Isoenzymes (genetics), Phanerochaete (enzymology), Phanerochaete (genetics), Reverse Transcriptase Polymerase Chain Reaction (methods).
- MESH :
- chemical , biosynthesis : Cellulase, Isoenzymes.
- chemical , genetics : Cellulase, DNA Primers, Isoenzymes.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- Gene Expression Profiling, Gene Expression Regulation, Fungal.
Abstract
Cellulolytic fungi generally secrete a cellulase mixture consisting mainly of glycoside hydrolase family 7 cellulases (Cel7s) during degradation of crystalline cellulose. Although several Cel7s have been investigated so far, the marked similarity in their amino acid and nucleotide sequences makes independent quantitative analysis difficult. Here, we present a real-time PCR method for the detection and quantification of Cel7 genes (cel7A-F/G) in the basidiomycete Phanerochaete chrysosporium using PCR primer sets designed based on the 3' untranslated region sequences. It was confirmed by agarose gel electrophoresis, sequencing, and dissociation curve analysis of the PCR products that each cel7 transcript was specifically amplified by the corresponding primers. We applied this real-time reverse-transcription PCR method using the presented primer sets to evaluate quantitatively the expression changes of cel7 genes in P. chrysosporium under conditions of carbon catabolite derepression.
DOI: 10.1111/j.1574-6968.2009.01753.x
PubMed: 19709307
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Kiyohiko" sort="Igarashi, Kiyohiko" uniqKey="Igarashi K" first="Kiyohiko" last="Igarashi">Kiyohiko Igarashi</name></author><author><name sortKey="Samejima, Masahiro" sort="Samejima, Masahiro" uniqKey="Samejima M" first="Masahiro" last="Samejima">Masahiro Samejima</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19709307</idno><idno type="pmid">19709307</idno><idno type="doi">10.1111/j.1574-6968.2009.01753.x</idno><idno type="wicri:Area/Main/Corpus">000602</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000602</idno><idno type="wicri:Area/Main/Curation">000602</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000602</idno><idno type="wicri:Area/Main/Exploration">000602</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Quantitative transcriptional analysis of the genes encoding glycoside hydrolase 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(MeSH)</term><term>Cellulase (biosynthèse)</term><term>Cellulase (génétique)</term><term>Isoenzymes (biosynthèse)</term><term>Isoenzymes (génétique)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>RT-PCR (méthodes)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cellulase</term><term>Isoenzymes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cellulase</term><term>DNA Primers</term><term>Isoenzymes</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cellulase</term><term>Isoenzymes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term><term>Cellulase</term><term>Isoenzymes</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>RT-PCR</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Fungal</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Régulation de l'expression des gènes fongiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cellulolytic fungi generally secrete a cellulase mixture consisting mainly of glycoside hydrolase family 7 cellulases (Cel7s) during degradation of crystalline cellulose. Although several Cel7s have been investigated so far, the marked similarity in their amino acid and nucleotide sequences makes independent quantitative analysis difficult. Here, we present a real-time PCR method for the detection and quantification of Cel7 genes (cel7A-F/G) in the basidiomycete Phanerochaete chrysosporium using PCR primer sets designed based on the 3' untranslated region sequences. It was confirmed by agarose gel electrophoresis, sequencing, and dissociation curve analysis of the PCR products that each cel7 transcript was specifically amplified by the corresponding primers. We applied this real-time reverse-transcription PCR method using the presented primer sets to evaluate quantitatively the expression changes of cel7 genes in P. chrysosporium under conditions of carbon catabolite derepression.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19709307</PMID><DateCompleted><Year>2009</Year><Month>11</Month><Day>17</Day></DateCompleted><DateRevised><Year>2009</Year><Month>09</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1574-6968</ISSN><JournalIssue CitedMedium="Internet"><Volume>299</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Oct</Month></PubDate></JournalIssue><Title>FEMS microbiology letters</Title><ISOAbbreviation>FEMS Microbiol Lett</ISOAbbreviation></Journal><ArticleTitle>Quantitative transcriptional analysis of the genes encoding glycoside hydrolase family 7 cellulase isozymes in the basidiomycete Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>159-65</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1574-6968.2009.01753.x</ELocationID><Abstract><AbstractText>Cellulolytic fungi generally secrete a cellulase mixture consisting mainly of glycoside hydrolase family 7 cellulases (Cel7s) during degradation of crystalline cellulose. Although several Cel7s have been investigated so far, the marked similarity in their amino acid and nucleotide sequences makes independent quantitative analysis difficult. Here, we present a real-time PCR method for the detection and quantification of Cel7 genes (cel7A-F/G) in the basidiomycete Phanerochaete chrysosporium using PCR primer sets designed based on the 3' untranslated region sequences. It was confirmed by agarose gel electrophoresis, sequencing, and dissociation curve analysis of the PCR products that each cel7 transcript was specifically amplified by the corresponding primers. 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